Friday, February 19, 2010


0 comentarios

Recent work at Washington University in St. Louis sheds light on one of the most important events in earth-history, the conquest of land by plants 480 million years ago.

No would-be colonizer could have survived on dry land without the ability to deal with dehydration, a major threat for organisms accustomed to soaking in water.

Clues to how the first land plants managed to avoid drying out might be provided by bryophytes, a group that includes the mosses, many of which retain remarkable drought tolerance. Some mosses can become so dry they crumble in the hand, but, if remoistened, will begin making proteins within minutes.

The work, published in the Jan. 29 issue of the journal Science, reveals several components of the signaling pathway that underlies the moss's Lazarus-like behavior.

Intriguingly, the flowering plants haven't entirely lost the ability to tolerate dessication: this tolerance has just been limited to their seeds, which dry out over winter and yet come to life in the spring.

"We began by asking whether the moss Physcomitrella patens, which we have been using as a model system, employs different molecules or different regulatory systems than seeds. Or does the same mechanism underlie its desiccation tolerance and that of seeds?" says Ralph Quatrano, Ph.D., the Spencer T. Olin Professor of biology at WUSTL and senior author on the paper.

Earlier, Quatrano and others had showed that seeds depended on both the plant hormone ABA and the regulatory molecule ABI3 to survive drying.

Plant hormones, much like human hormones, are chemicals produced in small amounts that have a profound effect on growth and development. The release of one plant hormone, for example, causes bolting, the sudden growth of a floral stalk that signals the end of a leaf lettuce's tasty days.

ABI3 is a transcription factor, a molecule that binds to a specific DNA sequence next to the gene it regulates and controls the copying of that gene into messenger RNA, the first step in making a protein from the gene.

"The assumption," says Quatrano, "was that in the presence of ABI3, ABA triggers some genes to make proteins and then when you dry out the seed, those gene products protect the cells."

In 1995 Quatrano and David Cove, Ph.D., a longtime colleague from the University of Leeds in the U.K., showed that ABA was at work in P. patens as well as in seeds.

In 2006 Heather Marella, a WUSTL graduate student, and Yoichi Sakata, Ph.D., a visiting scientist in Quatrano's lab, showed ABI3 is also present in P. patens. Whereas seed plants have only a single copy of the ABI3 gene, P. patens has at least three.

Marella and Sakata were able to delete the genes that code for ABI3 from the moss's genome. The resulting "knockout strains" would later prove useful in defining the separate roles of ABA and ABI3 in desiccation tolerance.

Technically, mosses tolerant to water stress can be either drought-tolerant or desiccation-tolerant. P. patens is drought-tolerant, able to withstand brief periods of water loss, but not desiccation-tolerant, that is not able to withstand total water loss.

Joseph Cho, Ph.D., a postdoctoral fellow from Korea in Quatrano's lab, showed that pre-treating P. patens with ABA toughened it up, making the drought-tolerant moss desiccation-tolerant. But pre-treatment with ABA didn't help the knockout mosses.

"So the first major conclusion of the paper," says Quatrano, "is that ABA alone cannot trigger desiccation tolerance. In mosses, as in seeds, both ABA and ABI3 are needed."

Next Quatrano's team set out to find the genes the ABA/ABI3 signaling pathway was controlling.

Abha Khandelwal, Ph.D., a postdoctoral fellow from India, identified 22 genes in wild-type moss that are upregulated and begin to churn out more protein product when the moss is treated with ABA.

"If you look at those genes," says Quatrano, "some are very similar to what you find in seeds. For example, some resemble the genes for the LEA (late embryogenesis abundant) proteins." These proteins, which accumulate late in seed development, are believed to protect seeds from cellular damage caused by water loss, such as the unfolding of proteins or the loss of membrane integrity.

Up until now everything had been playing out as expected. But then something unforeseen happened.

"We treated the knockout strains with ABA to see what would happen to gene expression," Quatrano says. "We thought that none of the genes would be expressed in these strains."

"To our surprise, all of them were upregulated."

"This didn't make a lot of sense," says Quatrano. "Why does the moss need ABI3 to become desiccation tolerant if only ABA is needed to upregulate the genes?"

"To solve this puzzle, we looked at rehydration separately from dehydration. Both wild-type and knockout moss express the 22 genes during dehydration, producing messenger RNA to begin protein manufacture. The wild-type moss also expresses the genes during rehydration.

"But when we rehydrated the knockout moss, all of the messenger RNA disappeared within 15 minutes," says Quatrano.

"This said to us that ABI3 either stabilizes the messenger RNA molecules whose creation is triggered by ABA, or it somehow allows messenger RNA to continue to be synthesized during rehydration."

In other words, ABI3 doesn't so much prepare tissue for desiccation as it helps it rehydrate after desiccation.

"So the second major conclusion of the research," says Quatrano, "was the timing of ABI3's role in desiccation tolerance."

"Our work showed ABA was able to confer desiccation tolerance on a moss that wasn't desiccation tolerant, but that it needed ABI3 to do that. We also found that ABA triggered genes that we assume are essential for desiccation tolerance, but that ABI3 does not play a role in preparing for dessication and is instead essential for the stability of the gene products after rehydration."

"Our hypothesis," says Quatrano, "is that the first plants to move onto land were desiccation tolerant like P. patens and the other bryophytes, such as the hornworts and liverworts. As vascular plants evolved, they abandoned desiccation tolerance in favor of adaptations such as extensive root systems, waxy cuticles and stomata, that would allow them to prevent water loss rather than simply to survive it." The genes that confer desiccation tolerance were not lost, however. Instead their expression was sequestered within spores or seeds.

Could these genes and the signaling pathway that controls them be reactivated in the vegetative tissue of the plants?

"Our goal," says Quatrano, "is to characterize the entire regulatory network that controls the desiccation-tolerance system. Once we understand that network, we might be able to 'engineer' it into the vegetative tissue of seed plants to make them more tolerant to water loss."

"But this is all very speculative," he adds.

Still the vision of sun-burned fields of corn blushing green when long-awaiting rain finally relieves a drought is exciting to contemplate.

(Photo: Hans Steur, Ellecom, The Netherlands)

Washington University in St. Louis


0 comentarios
Ancient human teeth are telling secrets that may relate to modern-day health: Some stressful events that occurred early in development are linked to shorter life spans.

"Prehistoric remains are providing strong, physical evidence that people who acquired tooth enamel defects while in the womb or early childhood tended to die earlier, even if they survived to adulthood," says Emory University anthropologist George Armelagos.

Armelagos led a systematic review of defects in teeth enamel and early mortality recently published in Evolutionary Anthropology. The paper is the first summary of prehistoric evidence for the Barker hypothesis – the idea that many adult diseases originate during fetal development and early childhood.

"Teeth are like a snapshot into the past," Armelagos says. "Since the chronology of enamel development is well known, it's possible to determine the age at which a physiological disruption occurred. The evidence is there, and it's indisputable."

The Barker hypothesis is named after epidemiologist David Barker, who during the 1980s began studying links between early infant health and later adult health. The theory, also known as the Developmental Origins of Health and Disease Hypothesis (DOHaD), has expanded into wide acceptance.

As one of the founders of the field of bioarcheology, Armelagos studies skeletal remains to understand how diet and disease affected populations. Tooth enamel can give a particularly telling portrait of physiological events, since the enamel is secreted in a regular, ring-like fashion, starting from the second trimester of fetal development. Disruptions in the formation of the enamel, which can be caused by disease, poor diet or psychological stress, show up as grooves on the tooth surface.

Armelagos and other bioarcheologists have noted the connection between dental enamel and early mortality for years. For the Evolutionary Biology paper, Armelagos led a review of the evidence from eight published studies, applying the lens of the Barker hypothesis to remains dating back as far as 1 million years.

One study of a group of Australopithecines from the South African Pleistocence showed a nearly 12-year decrease in mean life expectancy associated with early enamel defects. In another striking example, remains from Dickson Mounds, Illinois, showed that individuals with teeth marked by early life stress lived 15.4 years less than those without the defects.

"During prehistory, the stresses of infectious disease, poor nutrition and psychological trauma were likely extreme. The teeth show the impact," Armelagos says.

Until now, teeth have not been analyzed using the Barker hypothesis, which has mainly been supported by a correlation between birth weight in modern-day, high-income populations and ailments like diabetes and heart disease.

"The prehistoric data suggests that this type of dental evidence could be applied in modern populations, to give new insights into the scope of the Barker hypothesis," Armelagos says. "Bioarcheology is yielding lessons that are still relevant today in the many parts of the world in which infectious diseases and under-nutrition are major killers."

Emory University


0 comentarios
An ultra-lightweight sponge made of clay and a bit of high-grade plastic draws oil out of contaminated water but leaves the water behind.

And, lab tests show that oil absorbed can be squeezed back out for use.

Case Western Reserve University researchers who made the material, called an aerogel, believe it will effectively clean up spills of all kinds of oils and solvents on factory floors and roadways, rivers and oceans.

The EPA estimates that 10 to 25 million gallons of oil are spilled annually in this country alone. Spilled oil ruins drinking water, is a fire and explosion hazard, damages farmland and beaches and destroys wildlife and habitats. The harm can last decades.

The aerogel is made by mixing clay with a polymer and water in a blender, said David Schiraldi, chairman of the Macromolecular Science and Engineering department at the Case School of Engineering.

The mixture is then freeze-dried; air fills the gaps left by the loss of water. The resulting material is super light, comprised of about 96 percent air, 2 percent polymer and 2 percent clay.

The oil-absorbing form is just one of a growing list of clay-based aerogels being made in Schiraldi's lab. By adding different polymers, they produce materials with different properties.

"This particular one is oleophilic or oil-loving," Schiraldi said. "Chemically, it hates water, loves oil: the perfect combination."

The aerogel can be made in granular form, in sheets or in blocks of almost any shape and is effective in fresh and saltwater or on a surface. Because absorption is a physical phenomenon, there is no chemical reaction between the material and oil. If the oil is otherwise not contaminated, it can be used.

Oil spill experts on both coasts say that the ability to squeeze out and conserve the oil is an advantage over other products currently available.

The material was first made when Schiraldi challenged his then-PhD student Matt Gawryla with idea of making kitty litter. Gawryla added the oil cleanup concept to the program.

Case Western Reserve has granted a 9-month exclusive license for this and other clay-based aerogel technologies to AeroClay, Inc. a startup company. Schiraldi will be chief scientific officer of the new company.

Case Western Reserve University




Selected Science News. Copyright 2008 All Rights Reserved Revolution Two Church theme by Brian Gardner Converted into Blogger Template by Bloganol dot com